IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 170 
Small font sizeDefault font sizeIncrease font size
Navigate Here
 »   Next article
 »   Previous article
 »   Table of Contents

Resource Links
 »   Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »Related articles
 »   Citation Manager
 »   Access Statistics
 »   Reader Comments
 »   Email Alert *
 »   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed4437    
    Printed203    
    Emailed6    
    PDF Downloaded322    
    Comments [Add]    
    Cited by others 8    

Recommend this journal

 

 RESEARCH PAPER
Year : 2006  |  Volume : 38  |  Issue : 4  |  Page : 260-265

The effects of cyclophosphamide alone and in combination with ascorbic acid against murine ascites Dalton's lymphoma


Cell and Tumor Biology Laboratory, Department of ZoologyNorth-Eastern Hill University, Shillong-793 022, India

Correspondence Address:
S B Prasad
Cell and Tumor Biology Laboratory, Department of ZoologyNorth-Eastern Hill University, Shillong-793 022
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.27022

Rights and Permissions

Objective : To evaluate the therapeutic activity of cyclophosphamide alone and in combination with ascorbic acid against murine ascites Dalton's lymphoma. Materials and Methods: Cyclophosphamide (CP) is an anticancer drug with immunosuppressive activity, while ascorbic acid (AA) is an antioxidant. Ascites Dalton's lymphoma (DL) was maintained by intraperitoneal (i.p.) transplantation of tumor cells in Swiss albino mice. Tumor transplanted mice were divided into four groups. Group-I mice received normal saline only and served as control. Group-II mice were given 1% ascorbic acid through drinking water from the 5th to the 10th day. Group-III mice were injected i.p. with a single dose of CP (200 mg/kg) on the 10th day of tumor transplantation. Group IV mice received 1% ascorbic acid from the 5th day onwards and, then, a single dose of CP, i.p., on the 10th day of tumor transplantation. In groups III and IV, after 24, 48, 72, and 96 h of CP treatment the liver, kidneys, spleen, and tumor tissue were collected for biochemical determinations. In group II, which received AA only from the 5th to the 10th day, the same tissues were collected on the 10th day of tumor transplantation. The changes in reduced glutathione (GSH) and carbohydrate in tumor cells as well as the liver, kidney, and spleen of tumor-bearing mice in relation to the antitumor activity of CP alone or in combination with AA were evaluated. The quantitative changes in sialic acid level of DL cells under these treatment conditions were also determined. Results: AA and CP combination in tumor-bearing mice was found to be more effective against DL as it caused a 257% increase in life span compared with control, while it was 106% with AA and 188% with CP alone (ANOVA, P < 0.001). The reduced glutathione (GSH) level increased in DL cells with tumor growth. Compared with CP alone, the combination treatment (AA + CP) resulted in a more pronounced effect causing decreases in non-protein thiol (NPSH) as well as sialic acid levels in DL cells (ANOVA, P < 0.001). Conclusion: The drug-mediated lowering of GSH levels in DL cells may be involved in the cytotoxicity due to CP (group-III) as well as AA + CP combination (group-IV). An overall decrease in the sialic acid content of DL cells after combination treatment may also play a role to bring about alterations in the tumor cells, cell-cell interaction and enhanced tumor regression.






[FULL TEXT] [PDF]*


        
Print this article     Email this article

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow