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| RESEARCH PAPER
|Year : 2006 | Volume
| Issue : 1 | Page : 49-53
Antihyperglycemic activity of aqueous extract of leaves of Cocculus hirsutus (L.) Diels in alloxan-induced diabetic mice.
S Badole1, N Patel1, S Bodhankar1, B Jain1, S Bhardwaj2
1 Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane,Pune-411 038, India
2 Clinical Research, Serum Institute of India, Hadpsar, Pune-411 007, India
Objective: To evaluate the antihyperglycemic activity of aqueous extract of leaves of Cocculus hirsutus (L.) Diels in alloxan-induced diabetic mice. Materials and Methods: Alloxan-induced (70 mg/kg, i.v.) diabetic mice were given aqueous leaf extract (250, 500, and 1000 mg/kg, p.o., n= 6) of C. hirsutus or vehicle (distilled water, 10 ml/kg, p.o.) or standard drug glyburide (10 mg/kg, p.o.) for 28 days. Blood samples were withdrawn by retro-orbital puncture and were analyzed for serum glucose on 0th, 7th, 14th, 21st, and 28th days by glucose oxidase/peroxidase method. In oral glucose tolerance test, glucose (2.5 g/kg, p.o.) was administered to nondiabetic control, glyburide (10 mg/kg, p.o.), and aqueous extract of C. hirsutus (1000 mg/kg, p.o.) treated mice. The serum glucose level was analyzed at 0, 30, 60, and 120 min after drug administration. Results: The aqueous leaf extract of C. hirsutus (250, 500, and 1000 mg/kg, p.o.) showed significant ( P< 0.01) reduction of serum glucose level in alloxan-induced diabetic mice at 28th day. In oral glucose tolerance test, aqueous extract of C. hirsutus increased the glucose tolerance. Conclusion: It is concluded that C. hirsutus has significant antihyperglycemic activity as it lowers serum glucose level in diabetic mice and significantly increases glucose tolerance.
Department of Pharmacology, Poona College of Pharmacy, Bharati Vidyapeeth Deemed University, Erandwane,Pune-411 038
Source of Support: None, Conflict of Interest: None
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