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 RESEARCH PAPER
Year : 2006  |  Volume : 38  |  Issue : 1  |  Page : 33-37

Effects of Mondia whitei extracts on the contractile responses of isolated rat vas deferens to potassium chloride and adrenaline


1 Animal Physiology and Phytopharmacology Laboratory, Faculty of Science, University of Dschang, Box 67 Dschang, Cameroon
2 Department of Chemistry, Faculty of Science, University of Yaounde I, Box 812, Yaounde, Cameroon
3 Animal Physiology Laboratory, Faculty of Science, University of Yaounde I, Box 812, Yaounde, Cameroon

Correspondence Address:
P Watcho
Animal Physiology and Phytopharmacology Laboratory, Faculty of Science, University of Dschang, Box 67 Dschang
Cameroon
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Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.19850

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Objective: To investigate the effects of the methylene chloride:methanol (CH2Cl2:MeOH, 1:1) extract of the dried roots of Mondia whitei Linn and its hexane and methanol fractions on potassium chloride (KCl) and adrenaline (Adr)-induced contractions of rat vas deferens. Materials and Methods: Isolated strips of normal adult rat vas deferens were mounted in a Ugo Basile single-organ bath containing Krebs solution. Cumulative concentration-response curves of KCl (1-7 x 10-2 M ) and adrenaline (1.21-8.45 x 10-7 M ) were established in the absence and presence of M. whitei (50-400 g/ml). In separate experiments, after obtaining a stable plateau of contractions with KCl (60 m M ), M. whitei samples (50-400 g/ml) were added cumulatively to relax the preparation. In KCl (60 m M ), containing depolarizing medium, cumulative concentration-contraction curve to CaCl2 (2-14 x 10-2 M ) was elicited in the absence and presence of the hexane fraction of M. whitei (50-400 g/ml). Results: All the M. whitei samples produced rightward shift of the concentration-response curves to KCl and Adr. At high concentration of the plant extracts (400 g/ml), a decrease of the maximal response to the contractile agents was observed compared with that obtained with the control. All the three extracts produced concentration-dependent relaxation of the plateau of contraction induced by KCl and the hexane fraction appeared to be the more potent. In calcium-free physiological salt solution, the hexane fraction of M. whitei produced rightward shift to the concentration-response curve to CaCl2 and completely abolished the contractile effect of calcium at high concentration (400 g/ml). Conclusion: It is concluded that M. whitei extracts antagonized the contractile responses to KCl and Adr in isolated rat vas deferens, which could be due to the blockade of voltage-operated calcium channels.






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