lexi
IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 402 
Small font sizeDefault font sizeIncrease font size
Navigate Here
  Search
 
 » Next article
 » Previous article 
 » Table of Contents
  
Resource Links
 »  Similar in PUBMED
 »  Search Pubmed for
 »  Search in Google Scholar for
 »  Article in PDF (126 KB)
 »  Citation Manager
 »  Access Statistics
 »  Reader Comments
 »  Email Alert *
 »  Add to My List *
* Registration required (free)

 
In This Article
 »  References
 »  Article Tables

 Article Access Statistics
    Viewed15292    
    Printed265    
    Emailed14    
    PDF Downloaded764    
    Comments [Add]    
    Cited by others 39    

Recommend this journal

 


 
RESEARCH LETTER
Year : 2005  |  Volume : 37  |  Issue : 6  |  Page : 408-409
 

Preliminary screening of some folklore medicinal plants from western India for potential antimicrobial activity


Phytochemical, Pharmacological and Microbiological Laboratory, Department of Biosciences, Saurashtra University,Rajkot-360 005., India

Correspondence Address:
C Sumitra
Phytochemical, Pharmacological and Microbiological Laboratory, Department of Biosciences, Saurashtra University,Rajkot-360 005.
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/0253-7613.19085

Rights and Permissions



How to cite this article:
Jigna P, Rathish N, Sumitra C. Preliminary screening of some folklore medicinal plants from western India for potential antimicrobial activity. Indian J Pharmacol 2005;37:408-9

How to cite this URL:
Jigna P, Rathish N, Sumitra C. Preliminary screening of some folklore medicinal plants from western India for potential antimicrobial activity. Indian J Pharmacol [serial online] 2005 [cited 2022 May 16];37:408-9. Available from: https://www.ijp-online.com/text.asp?2005/37/6/408/19085


In recent years, multiple drug resistance has developed due to indiscriminate use of existing antimicrobial drugs in the treatment of infectious diseases.[1] In addition to this, antibiotics are sometimes associated with adverse effects on the host-like hypersentivity. Therefore, there is a need to develop alternative antimicrobial drugs for the treatment of infectious diseases from other sources, such as plants.[2] Natural products of higher plants may be a new source of antimicrobial agents possibly with novel mechanisms of action.[3]

In vitro antimicrobial activity of aqueous and ethanol extracts of six medicinal plants used by traditional healers was examined. Microorganisms were obtained from National Chemical Laboratory (NCL), Pune, India. Microorganisms were maintained at 4°C on nutrient agar slants (for bacteria) and MGYP slants (for yeast).

Plant material (10 g) was extracted with 100 ml ethanol by subjecting it to agitation on rotary shaker (190-200 rpm) overnight, filtering it with muslin cloth and concentrating it to one-fifth of the volume. Crude aqueous extract was prepared by subjecting plant material (10 g) to slow heat for 6 hours and filtered through muslin cloth and concentrated it to one-fifth of the total volume. Antimicrobial activity of the ethanolic extracts was evaluated by agar well diffusion method[4] while aqueous extracts were evaluated by agar disc diffusion method.[5] The sensitivity of the extracts was measured using the diameter of the zone of inhibition.

Amongst the 12 extracts obtained from six plants, majority were active against Gram-positive bacteria [Table - 1], of which, the ethanol extracts were more active than aqueous extracts. The aqueous extract of Acyranthus aspera L. could inhibit Staphylococcus epidermidis to a certain extent; while rest of the bacteria was resistant to both aqueous and ethanolic extract of this plant. Aqueous extract of Calotropis gigantea L. could not inhibit any of the bacterial strains investigated except Proteus mirabilis. The ethanolic extract was most active against Klebsiella pneumoniae. Carissa congesta Wt. showed similar trend as that of Calotropis gigantea L. in which aqueous extract showed some activity only against Bacillus cereus, while ethanol extract could show antimicrobial activity to some extent. The ethanolic extract of Fagonia cretica L. and Rauvolfia serpentina L. could inhibit some of the bacterial strains studied to some degree while aqueous extract of both these plants did not show any activity at all. M. indica L. (both extracts) displayed remarkable activity. The most susceptible bacteria were Klebsiella pneumoniae Scientific Name Search  followed by Bacillus cereus,  Escherichia More Details coli and Alcaligenes fecalis.  Salmonella More Details typhimurium was the most resistant bacteria. All the aqueous extracts were inactive against C. tropicalis and only three ethanolic extracts viz. Calotropis gigantea L., Carissa congesta Wt. and Rauwolfia serpentina L. showed anticandidal activity . These observations are likely to be the result of the differences in cell wall structure between Gram-positive and Gram-negative bacteria, with Gram-negative outer membrane acting as a barrier to many environmental substances, including antibiotics.[6] The results can be compared with the standard antimicrobials [Table - 1].

In conclusion, the results of the present study support the folkloric usage of the studied plants and suggest that some of the plant extracts possess compounds with antimicrobial properties that can be further explored for antimicrobial activity.

 
 » References Top

1.Service RF. Antibiotics that resist resistance. Science 1995;270:724-7.  Back to cited text no. 1  [PUBMED]  
2.Cordell GA. Biodiversity and drug discovery a symbiotic relationship . Phytochemistry 2000;55:463-80.  Back to cited text no. 2  [PUBMED]  [FULLTEXT]
3.Barbour EK, Al Sharif M, Sagherian VK, Habre AN, Talhouk RS, Talhouk SN. Screening of selected indigenous plants of Lebanon for antimicrobial activity. J Ethnopharmacol 2004;93:1-7.  Back to cited text no. 3  [PUBMED]  [FULLTEXT]
4.Perez C, Paul M, Bazerque P. Antibiotic assay by agar-well diffusion method. Acta Biol Med Exp 1990;15:113-5.  Back to cited text no. 4    
5.Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 1966;45:493-6.  Back to cited text no. 5  [PUBMED]  
6.Tortora GJ, Funke BR, Case CL. Microbiology: An Introduction. San Francisco: Benjamin Cummings; 2001.  Back to cited text no. 6    


Tables

[Table - 1]

This article has been cited by
1 Antimicrobial and cytotoxic potential of an endophytic fungus Alternaria tenuissima AUMC14342 isolated from Artemisia judaica L. growing in Saudi Arabia
Amal A. Al Mousa, Hassan Mohamed, Abdallah M.A. Hassane, Nageh F. Abo-Dahab
Journal of King Saud University - Science. 2021; 33(5): 101462
[Pubmed] | [DOI]
2 In Vitro Cytotoxic Evaluation and Apoptotic Effects of Datura innoxia Grown in Saudi Arabia and Phytochemical Analysis
Mohammed Al-Zharani, Fahd A. Nasr, Ali S. Alqahtani, Mary Anne W. Cordero, Amal A. Alotaibi, Asmatanzeem Bepari, Saud Alarifi, Ali Daoud, Ibrahim O. Barnawi, Haytham M. Daradka
Applied Sciences. 2021; 11(6): 2864
[Pubmed] | [DOI]
3 Saussurea lappa Exhibits Anti-Oncogenic Effect in Hepatocellular Carcinoma, HepG2 Cancer Cell Line by Bcl-2 Mediated Apoptotic Pathway and Mitochondrial Cytochrome C Release
Amal A. Alotaibi, Asmatanzeem Bepari, Rasha Assad Assiri, Shaik Kalimulla Niazi, Sreenivasa Nayaka, Muthuraj Rudrappa, Shashiraj Kareyellapa Nagaraja, Meghashyama Prabhakara Bhat
Current Issues in Molecular Biology. 2021; 43(2): 1114
[Pubmed] | [DOI]
4 In Vitro Synergy of Pongamia pinnata Extract in Combination with Antibiotics for Inhibiting and Killing Methicillin-Resistant Staphylococcus aureus
Po-An Su, Shun-Lai Li, Hung-Jen Tang, Chi-Chung Chen, Ying-Chen Lu, Kuo-Chen Cheng, Yi-Chung Lin, Yin-Ching Chuang, Chih-Cheng Lai
Antibiotics. 2020; 9(3): 103
[Pubmed] | [DOI]
5 Xanthine Oxidase Inhibition, In Vitro Antioxidant Activity and Antibacterial Effect of the Aerial Part Extracts of Thymus pallidus Coss
Abderrahim Benslama, Sabah Boumerfeg, Sana Aouachria, Thoraya Guemmaz, Lekhmici Arrar, Seddik Khennouf, Abderrahmene Baghiani
Current Nutrition & Food Science. 2020; 16(4): 607
[Pubmed] | [DOI]
6 Antimicrobial activity of rosemary leaf extracts and efficacy of ethanol extract against testicular damage caused by 50-Hz electromagnetic field in albino rats
Hanaa S. S. Gazwi, Magda E. Mahmoud, Moaz M. Hamed
Environmental Science and Pollution Research. 2020; 27(13): 15798
[Pubmed] | [DOI]
7 Phytochemical screening, thin-layer chromatography and antimicrobial activity study of Parquetina nigrescens leaf extracts
Sunday Ogakwu Adoga, Deborah Ehikowoicho Ekle, Barnabas Aloo Kyenge, Terkaa Ogo-Oluwa Aondo, Chris Oche Ikese
Ovidius University Annals of Chemistry. 2019; 30(2): 88
[Pubmed] | [DOI]
8 GC-MS ANALYSIS OF HEXANE: CHLOROFORM FRACTION OF INDIGOFERA TRITA L. F.
J. Aranganayaki, G. Jayanthi
INDIAN DRUGS. 2018; 55(02): 15
[Pubmed] | [DOI]
9 Antibacterial, antioxidant, and topical anti-inflammatory activities ofBergia ammannioides: A wound-healing plant
Shahira M. Ezzat,Mouchira A. Choucry,Zeinab A. Kandil
Pharmaceutical Biology. 2015; : 1
[Pubmed] | [DOI]
10 Physicochemical and biological properties of luteolin-7-O-ß-d-glucoside (cynaroside) isolated from Anthriscus sylvestris (L.) Hoffm.
Lukáš Žemlicka,Peter Fodran,Vladimír Lukeš,Adam Vagánek,Mariana Slováková,Andrej Staško,Tibor Dubaj,Tibor Liptaj,Michal Karabín,Lucia Birošová,Peter Rapta
Monatshefte für Chemie - Chemical Monthly. 2014;
[Pubmed] | [DOI]
11 Metaphase arrest and delay in cell cycle kinetics of root apical meristems and mouse bone marrow cells treated with leaf aqueous extract of Clerodendrum viscosum Vent
S. Ray, L. M. Kundu, S. Goswami, G. C. Roy, S. Chatterjee, S. Dutta, A. Chaudhuri, C. S. Chakrabarti
Cell Proliferation. 2013; 46(1): 109
[VIEW] | [DOI]
12 Investigating the chemical Composition and the antimicrobial activity of the essential oil and Crude Extracts of Sedum Microcarpum (Sm.) schönl Growing Wild in Jordan
Mahmoud A. Al-Qudah,Riyadh Muhaidat,Ahmed A. Alomary,Emad H. Malkawi,Hala I. Al-Jaber,Ibrahim N. Trawenh,Musa H. Abu Zarga,Sultan T. Abu Orabi
Pharmacognosy Journal. 2012; 4(33): 1
[Pubmed] | [DOI]
13 Antioxidant, Antimicrobial Properties and Phenolics of Different Solvent Extracts from Bark, Leaves and Seeds of Pongamia pinnata (L.) Pierre
Zahid Iqbal Sajid,Farooq Anwar,Ghulam Shabir,Ghulam Rasul,Khalid M. Alkharfy,Anwarul-Hassan Gilani
Molecules. 2012; 17(12): 3917
[Pubmed] | [DOI]
14 In vitro propagation and characterization of phenolic content along with antioxidant and antimicrobial activities of Cichorium pumilum Jacq.
Wesam Al Khateeb, Emad Hussein, Lolita Qouta, Muhammad Alu’datt, Baker Al-Shara, Ahmed Abu-zaiton
Plant Cell Tissue and Organ Culture. 2012;
[VIEW] | [DOI]
15 Screening of methanol & acetone extract for antimicrobial activity of some medicinal plants species of Indian folklore
Gami, B., Parabia, F.
International Journal of Research in Pharmaceutical Sciences. 2011; 2(1): 69-75
[Pubmed]
16 Preliminary phytochemical analysis and screening of antibacterial activity of Desmodium triflorum DC
Ajitha Rani, R., Sane, R.T., Vakil, B.V.
Indian Drugs. 2011; 48(1): 44-47
[Pubmed]
17 Antimicrobial Activity of <i>Terminalia catappa</i> Extracts Against Some Pathogenic Microbial Strains
Abul Manzur,Arifuddin Raju,Shahedur Rahman
Pharmacology & Pharmacy. 2011; 02(04): 299
[Pubmed] | [DOI]
18 Evaluation of antibacterial potential of Trikatu churna and its ingredients: An in vitro study
Dahikar, S.B., Bhutada, S.A., Vibhute, S.K., Sonvale, V.C., Tambekar, D.H., Kasture, S.B.
International Journal of Phytomedicine. 2010; 2(4): 412-417
[Pubmed]
19 In vitro antifungal activity of indirubin isolated from a South Indian ethnomedicinal plant Wrightia tinctoria R. Br.
Ponnusamy, K., Petchiammal, C., Mohankumar, R., Hopper, W.
Journal of Ethnopharmacology. 2010; 132(1): 349-354
[Pubmed]
20 The study of in vitro antimicrobial activity and phytochemical analysis of some medicinal plants in Chamoli Garhwal Region
Chaudhary, S., Negi, A., Dahiya, V.
Pharmacognosy Journal. 2010; 2(12): 481-485
[Pubmed]
21 Rauvolfia serpentina (L). benth. ex kurz.-A review
Dey, A., De, J.N.
Asian Journal of Plant Sciences. 2010; 9(6): 285-298
[Pubmed]
22 Antimicrobial efficacy and tissue reaction of Euphorbia hirta ethanolic extract oncanine wounds
Akinrinmade, J.F., Oyeleye, O.A.
African Journal of Biotechnology. 2010; 9(31): 5028-5031
[Pubmed]
23 Antibacterial activity of plant extracts against plant bacterial pathogens
Arunachalam, P., Sankar, M., Subramanian, B.
Asian Journal of Microbiology, Biotechnology and Environmental Sciences. 2010; 12(1): 167-170
[Pubmed]
24 Triterpenoids from Psidium guajava with biocidal activity
Ghosh, P., Mandal, A., Chakraborty, P., Rasul, M.G., Chakraborty, M., Saha, A.
Indian Journal of Pharmaceutical Sciences. 2010; 72(4): 504-507
[Pubmed]
25 Phytochemical analysis and antibacterial screening of in vivo and in vitro extracts of indian medicinal herb: Anethum graveolens
Jana, S., Shekhawat, G.S.
Research Journal of Medicinal Plant. 2010; 4(4): 206-212
[Pubmed]
26 Antimicrobial activity of white and pink nelumbo nucifera gaertn flowers
Brindha, D., Arthi, D.
Journal of Pharmaceutical Research and Health Care. 2010; 2(2): 147-155
[Pubmed]
27 In vitro antibacterial activity of crude leaf extracts from Tecoma Stans (L) juss. et kunth, Coleus Forskohlii and Pogostemon Patchouli against human pathogenic bacteria
Senthilkumar, C.S., Kumar, M.S., Pandian, M.R.
International Journal of PharmTech Research. 2010; 2(1): 438-442
[Pubmed]
28 Antioxidant and antimicrobial properties of kaff maryam (Anastatica hierochuntica) and doum palm (Hyphaene thebaica)
Mohamed, A.A., Khalil, A.A., El-Beltagi, H.E.S.
Grasas y Aceites. 2010; 61(1): 67-75
[Pubmed]
29 In vitro antifungal activity of indirubin isolated from a South Indian ethnomedicinal plant Wrightia tinctoria R. Br.
Kannan Ponnusamy, Chelladurai Petchiammal, Ramasamy Mohankumar, Waheeta Hopper
Journal of Ethnopharmacology. 2010; 132(1): 349
[VIEW] | [DOI]
30 The Study of in vitro Antimicrobial Activity and Phytochemical Analysis of Some Medicinal Plants in Chamoli Garhwal Region
Chaudhary Sachin,Negi Arvind,Dahiya Vinesh
Pharmacognosy Journal. 2010; 2(12): 481
[Pubmed] | [DOI]
31 Antimicrobial and lipid peroxidation inhibition activity of Oxystelma esculentum (Asclepiadaceae)
Ashok Kumar D., Thamil Selvan V., Prerona Saha, Aminul Islam, Upal Kanti Mazumder, Malaya Gupta
Oriental Pharmacy and Experimental Medicine. 2010; 10(3): 208
[VIEW] | [DOI]
32 Phytochemical Analysis and Antibacterial Screening of in vivo and in vitro Extracts of Indian Medicinal Herb: Anethum graveolens
Sonali Jana,G.S. Shekhawat
Research Journal of Medicinal Plant. 2010; 4(4): 206
[Pubmed] | [DOI]
33 Antioxidant and antimicrobial properties of kaff maryam (Anastatica hierochuntica) and doum palm (Hyphaene thebaica)
Amal A. Mohamed,Ashraf A. Khalil,Hossam E. S. El-Beltagi
Grasas y Aceites. 2010; 61(1): 67
[Pubmed] | [DOI]
34 Phytochemical screening and antibacterial properties of leaves of Pongamia pinnata Linn. (Fabaceae) from India
Arote, S.R., Dahikar, S.B., Yeole, P.G.
African Journal of Biotechnology. 2009; 8(22): 6393-6369
[Pubmed]
35 A review: Carissa congesta: Phytochemical constituents, traditional use and pharmacological properties
Devmurari, V., Shivanand, P., Goyani, M.B., Vaghani, S., Jivani, N.P.
Pharmacognosy Reviews. 2009; 3(6): 375-377
[Pubmed]
36 Immunomodulatory properties of Indian medicinal plants in Kolli hills: Stimulation and suppression of lymphocyte proliferation - In vitro
Perinbam, K., Arokiyaraj, S., Mathew, A., Aysha, O.S., Balaraju, K., Agastian, P.
Biosciences Biotechnology Research Asia. 2008; 5(1): 193-199
[Pubmed]
37 Differential sensitivity of Bacillus sp. isolated from archive materials to plant extracts
Guiamet, P.S., de la Paz Naranjo, J., Arenas, P.M., Gómez de Saravia, S.G.
Pharmacologyonline. 2008; 3: 649-658
[Pubmed]
38 Antifeedant effect of Achyranthes aspera Linn on cauliflower borer (Hellula undalis), fruit and leaf borer of cauliflower (Spodoptera litura) and Brinjal fruit borer (Leucinodes arbonalis)
Girija, S., Valarmathy, N.
Biosciences Biotechnology Research Asia. 2008; 5(2): 663-672
[Pubmed]
39 In vitro antimicrobial activity and phytochemical analysis of some Indian medicinal plants
Parekh, J., Chanda, S.V.
Turkish Journal of Biology. 2007; 31(1): 53-58
[Pubmed]



 

Top
Print this article  Email this article

    

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer | Privacy Notice
Online since 20th July '04
Published by Wolters Kluwer - Medknow