IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 22799 
Small font sizeDefault font sizeIncrease font size
Navigate Here
 ╗   Next article
 ╗   Previous article
 ╗   Table of Contents

Resource Links
 ╗   Similar in PUBMED
 ╗  Search Pubmed for
 ╗  Search in Google Scholar for
 ╗Related articles
 ╗   Citation Manager
 ╗   Access Statistics
 ╗   Reader Comments
 ╗   Email Alert *
 ╗   Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed6396    
    Printed247    
    Emailed8    
    PDF Downloaded232    
    Comments [Add]    
    Cited by others 9    

Recommend this journal

 

 RESEARCH PAPER
Year : 2004  |  Volume : 36  |  Issue : 4  |  Page : 238-243

Morphological and biochemical basis of centchroman as a novel antineoplastic agent in MCF-7 human breast cancer cells


Tissue Culture Laboratory, National Laboratory Animal Centre, Central Drug Research Institute, Lucknow - 226 001, India

Correspondence Address:
Anil K Balapure
Tissue Culture Laboratory, National Laboratory Animal Centre, Central Drug Research Institute, Lucknow - 226 001
India
Login to access the Email id

Source of Support: None, Conflict of Interest: None


Rights and PermissionsRights and Permissions

OBJECTIVE: To investigate the antineoplastic potential of a novel antiestrogen (AE) centchroman (CENT) using cell morphology, viability and cathepsin-D (Cath-D) gene expression as indices in MCF-7 human breast cancer cell line. MATERIAL AND METHODS: MCF-7 cells were incubated with CENT (1 ÁM, 10 ÁM) in the absence and presence of 17- estradiol (E2, 10 nM) for 4 days. Investigations were carried out on alterations in cell morphology, number, Cath-D gene expression using Phase Contrast Microscopy, Trypan-blue dye exclusion and spectrophotometric assay. A standard antiestrogen and antibreast cancer agent tamoxifen (TAM -1 ÁM, 10 ÁM) was used as a positive control. RESULTS: E2 (10 nM) alone significantly up-regulated the cells morphologically, numerically and biochemically thus establishing their hormonal responsiveness. TAM and CENT at 1 ÁM each enhanced and reduced the viable cell number respectively whereas both elevated the Cath-D activity. However, 35mm photographic evidence did not indicate any cytotoxic effect with either drug as compared to control. Addition of E2 to the two ligands separately affected the three parameters similar to that in E2 alone. With 10 ÁM TAM and CENT individually, the cells were visually devastated and so were the viable cell numbers and Cath-D gene expression. Supplementation of E2 under similar conditions failed to undo the damage to the cells morphologically and quantitatively. However, disparate results were observed with regards to the enzyme activity where TAM+E2 caused inhibition and CENT+E2 resulted in an induction. CONCLUSION: It can be concluded that both the AEs TAM and CENT displayed similarities in their antineoplastic action on MCF-7 cells as observed by morphological and viability studies except that the latter proved cytotoxic even at 1ÁM concentration. TAM and CENT showed a similar effect on Cath-D gene expression except at the higher dose (10 ÁM). These results suggest that the antiestrogen CENT has better antineoplastic potential as opposed to TAM.






[FULL TEXT] [PDF]*


        
Print this article     Email this article

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer
Online since 20th July '04
Published by Wolters Kluwer - Medknow