| RESEARCH PAPER
|Year : 1999 | Volume
| Issue : 6 | Page : 440-441
Bioassay of acetylcholine with horizontal strips of rat fundus
Sa SB De, Sa SB De, VG Dhume
Objective: To find out the suitability of horizontal strips of rat fundus for bioassay of acetylcholine (ACh).
Methods: Dose response curves to ACh were obtained using a horizontal strip of rat fundus suspended in Tyrode solution. The effects of histamine (H) and 5-hydroxytryptamine (5-HT) were also studied.
Results: Horizontal strips of rat fundus were stable over a long period of time (more than 6 hrs) showing reproducible responses to Ach with good discrimination between doses. It did not respond to H and 5-HT. Thus it was specific to ACh, the sensitivity being 10 -8 g/ml.
Conclusion: The modified preparation of rat fundus described by us is simple and satisfies the prerequisites for the bioassay of ACh.
Sa SB De
Source of Support: None, Conflict of Interest: None