| RESEARCH PAPER
|Year : 1999 | Volume
| Issue : 4 | Page : 275-278
Effect of therapeutics strategy established by N-acetyl cysteine and vitamin C on the activities of tumour marker enzymes in vitro
AJ Vanisree, Devi CS Shyamala
Objectives: To assess the therapeutic efficiency of N-acetylcysteine (NAC) and Vitamin C (VC) by estimating the activities of tumour marker enzymes in vitro.
Methods: Human lung carcinoma cell line, NCI-H82, was maintained in RPMI-1640 medium. The cells were divided into three groups (group II, III and IV) and each monolayer was subjected to drug treatment with NAC, VC and NAC+VC respectively. The cells without any supplementation served as control (group I). After 48 hours supplementation with the drugs, the cells were harvested for the estimation of aldolase, (-GT, 5'-nucleotidase, adenosine deaminase, lactate dehydrogenase and creatine kinase.
Results: The activities of aldolase, (-GT, 5'-nucleotidase, lactate dehydrogenase and creatine kinase were found to be altered but no change could be observed in the activity of adenosine deaminase.
Conclusion: The enzymes studied were found to be affected by the action of N-acetylcysteine and vitamin C, and this effect of the drugs on enzymes might contribute to the drugs action against cancer cells.
A J Vanisree
Source of Support: None, Conflict of Interest: None