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 RESEARCH PAPER
Year : 1998  |  Volume : 30  |  Issue : 4  |  Page : 263-266

Simple spectrofluorometric and microbiological assay methods for the estimation of ofloxacin in biological fluids



Correspondence Address:
Gurumurthy Prema


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Source of Support: None, Conflict of Interest: None


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Objective: To evolve simple methods for the assay of ofloxacin in biological fluids. Methods: Simple methods for the estimation of ofloxacin in plasma, saliva and urine employing micro-biological assay using plate diffusion technique and by fluorimetric method based on the measurement of native fluorescence emitted by ofloxacin, have been described. Results: The recovery of ofloxacin from all the three biologial fluids was 93-98% and the sensitivity was 0.5 'g/ml on all 5 different occasions by both the methods. Anti-TB drugs viz., rifampicin, ethambutol, isoniazid and pyrazinamide and also anti-leprosy drugs viz., dapsone and clofazimine at concentrations of 10 and 20 'g/ml did not interfere with the estimation of ofloxacin by either method. Ofloxacin is stable in biological fluids for a period of at least 8 days at -20° C. Conclusion: Both the methods described are simple, involve very few steps and do not need either costly chemicals or sophisticated equipments.






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