| RESEARCH PAPER
|Year : 1998 | Volume
| Issue : 4 | Page : 263-266
Simple spectrofluorometric and microbiological assay methods for the estimation of ofloxacin in biological fluids
Gurumurthy Prema, Ramachandran Geetha, AK Hemanthkumar, P Venkatesan, V Chandrasekaran, PR Narayanan
Objective: To evolve simple methods for the assay of ofloxacin in biological fluids.
Methods: Simple methods for the estimation of ofloxacin in plasma, saliva and urine employing micro-biological assay using plate diffusion technique and by fluorimetric method based on the measurement of native fluorescence emitted by ofloxacin, have been described.
Results: The recovery of ofloxacin from all the three biologial fluids was 93-98% and the sensitivity was 0.5 'g/ml on all 5 different occasions by both the methods. Anti-TB drugs viz., rifampicin, ethambutol, isoniazid and pyrazinamide and also anti-leprosy drugs viz., dapsone and clofazimine at concentrations of 10 and 20 'g/ml did not interfere with the estimation of ofloxacin by either method. Ofloxacin is stable in biological fluids for a period of at least 8 days at -20° C.
Conclusion: Both the methods described are simple, involve very few steps and do not need either costly chemicals or sophisticated equipments.
Source of Support: None, Conflict of Interest: None