IPSIndian Journal of Pharmacology
Home  IPS  Feedback Subscribe Top cited articles Login 
Users Online : 2601 
Small font sizeDefault font sizeIncrease font size
Navigate Here
 ╗   Next article
 ╗   Previous article
 ╗   Table of Contents

Resource Links
 ╗   Similar in PUBMED
 ╗  Search Pubmed for
 ╗  Search in Google Scholar for
 ╗Related articles
 ╗   Citation Manager
 ╗   Access Statistics
 ╗   Reader Comments
 ╗   Email Alert *
 ╗   Add to My List *
 * Requires registration (Free)

 Article Access Statistics
    PDF Downloaded289    
    Comments [Add]    

Recommend this journal


Year : 1998  |  Volume : 30  |  Issue : 4  |  Page : 263-266

Simple spectrofluorometric and microbiological assay methods for the estimation of ofloxacin in biological fluids

Correspondence Address:
Gurumurthy Prema

Login to access the Email id

Source of Support: None, Conflict of Interest: None

Rights and PermissionsRights and Permissions

Objective: To evolve simple methods for the assay of ofloxacin in biological fluids. Methods: Simple methods for the estimation of ofloxacin in plasma, saliva and urine employing micro-biological assay using plate diffusion technique and by fluorimetric method based on the measurement of native fluorescence emitted by ofloxacin, have been described. Results: The recovery of ofloxacin from all the three biologial fluids was 93-98% and the sensitivity was 0.5 'g/ml on all 5 different occasions by both the methods. Anti-TB drugs viz., rifampicin, ethambutol, isoniazid and pyrazinamide and also anti-leprosy drugs viz., dapsone and clofazimine at concentrations of 10 and 20 'g/ml did not interfere with the estimation of ofloxacin by either method. Ofloxacin is stable in biological fluids for a period of at least 8 days at -20° C. Conclusion: Both the methods described are simple, involve very few steps and do not need either costly chemicals or sophisticated equipments.


Print this article     Email this article

Site Map | Home | Contact Us | Feedback | Copyright and Disclaimer | Privacy Notice
Online since 20th July '04
Published by Wolters Kluwer - Medknow