| RESEARCH PAPER
|Year : 1997 | Volume
| Issue : 6 | Page : 405-410
Molecular genetic markers predict development of drug-induced leukemias in patients undergoing cytotoxic therapy
Kumaravel Bharathy, TS Kumaravel, Gajendiran, N Mary, N Mohankumar, Arif Mansyur
Objective: To demonstrate the usefulness of fluorescence in situ hybridization(FISH) directed against monosomy 7 and 5q-, in early detection of therapy related acute myeloid leukemia (t-AML) in patients receiving cytotoxic chemotherapy.
Methods: This is a retrospective study of five patients who developed t-AML/t-MDS after cytotoxic chemotherapy for primary malignant disorders. These patients underwent periodic cytogenetic work-up and the remaining samples were available for FISH analysis. Clinical and cytogenetic data concerning these patients were retrieved from the records of the Clinical Pathology Department. Sequential interphase FISH analysis with D7Z1 and 5q31.1 probe was performed as per standard protocols to detect the exact time of origin of these abnormalities.
Results: FISH detected monosomy 7 and 5q-atleast 12-36 months before the onset of clinical manifestations in these patients.
Conclusion: Molecular cytogenetic techniques can be of good use to clinical pharmacologists in early detection of t-AML in patients undergoing cytotoxic chemotherapy.
Source of Support: None, Conflict of Interest: None