| SHORT COMMUNICATION
|Year : 1997 | Volume
| Issue : 4 | Page : 262-265
Effect of dietary fat on tissue disposition of clofazimine in mice receiving the drug orally as suspension in oils
A Mathur, K Venkatesan, Sreevatsa, KB Kannan, KV Desikan
Objective: To evaluate the effect of dietary fats on the tissue disposition of clofazimine in mice receiving the drug orally as suspension in oil(s).
Methods: Forty-five Balb/C mice were fed with either laboratory made fat-restricted diet or this diet supplemented to a fat content of 10% (w/w) with mustard oil-groundnut oil/vegetable ghee and administered clofazimine suspension in mustard oil/groundnut oil/lard oil orally at a daily dose of 50 'g for seven consecutive days. Four hours after the last daily dose the mice were sacrificed, tissues collected and clofazimine content estimated by spectrophotometric methods.
Results: The drug was found accumulated in greater amounts in mesentric lymph nodes, mesentric fat, lungs, spleen, liver, kidney and small intestine and to a lesser extent in heart, muscle and tail. Most tissues from mice fed with fat-supplemented diet presented significantly more levels of clofazimine (P<0.05). The mean ˝ SD cumulative tissue clofazimine content in mice fed with fat supplemented diet ranged from 41.2 ˝ 6.38 to 43.4 ˝ 2.10 'g/g while it ranged from 34.6 ˝ 3.16 to 37.7 ˝ 2.97 'g/g in mice fed with fat restricted diet. However, the variety of oil/fat used either in the diet or drug suspensions failed to make any significant difference in drug availability in tissues.
Conclusion: An extrapolation of these findings to human beings suggest that clofazimine may be administered to the patients along with or after any fatty meal for better absorption and tissue deposition.
Source of Support: None, Conflict of Interest: None