| RESEARCH PAPER
|Year : 1997 | Volume
| Issue : 3 | Page : 182-186
Modulation of in vitro anaphylaxis by furosemide
AM Nair, AA Mungantiwar, KM Kamal, MN Saraf
Objective: To study the effects of furosemide on the isolated guinea pig tracheal spirals in the presence of compound 48/80 and antigen (egg albumin).
Methods: Compound 48/80 and egg albumin were used to produce contractions of the non-sensitised and sensitised guinea pig trachea respectively. Furosemide was added in the bath 15 min prior to compound 48/80 or egg albumin challenge. In a separate set of experiments, the effects of furosemide were tested in the presence of indomethacin. Contractions were recorded for a period of 60 min.
Results: Furosemide at concentrations of 10-6M and 10-5M significantly attenuated the tracheal contractions induced by compound 48/80 and egg albumin and altered the time duration and the area under the time-contraction curve. The inhibitory effect of furosemide was partially reversed by the preincubation of the tissue with indomethacin (8.6 x 10-6M).
Conclusion: The studies reveal that furosemide may be causing a local release of PGE2 into the airways or could be affecting mediator release from mast cells and may thus be contributing to the observed effect.
A M Nair
Source of Support: None, Conflict of Interest: None