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Year : 1997  |  Volume : 29  |  Issue : 2  |  Page : 129-131

Effect of sobatum on ultra violet-induced damage and superoxide production

Correspondence Address:
P V Mohanan

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Source of Support: None, Conflict of Interest: None

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Objectives: To evaluate the in vitro effect of sobatum on UV light induced damage and the production of superoxides by the photoactivation of riboflavin. Methods: The inhibitory effect of sobatum on UV light induced free radicals was determined using the bacteria Salmonella typhimurium. In this assay different concentrations of the sobatum in O.1ml DMSO were incorporated into the nutrient agar plates and the bacteria (1O8 cells in 0.1 ml) were spread on all the petri dishes using a sterile loop and all the petridishes were irradiated. At the end of 24 hours, the number of colonies in each plate were counted and the increase in number of colonies in the sobatum incorporated petridishes were compared to the control petridish. Effect of sobatum on superoxide production was determined by its ability to inhibit the reduction of nitro blue tetrazolium (NBT) by superoxides generated by the reaction of photo reduced riboflavin and oxygen. Results: Sobatum significantly inhibited UV induced damage in Salmonella typhimurium. The inhibitory effect of sobatum on superoxide production by the photoactivation of riboflavin was about 79.40% (0.4mg sobatum) when compared with control. Conclusion: Sobatum inhibits the UV light induced damage due to its free radical scavenging property.


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