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Year : 1996  |  Volume : 28  |  Issue : 1  |  Page : 11-15

Isolation of opiate receptor enriched membranes by subcellular fractionation

Correspondence Address:
Shekar B Chandra

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Source of Support: None, Conflict of Interest: None

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The present study is aimed at developing a method by which opiate receptor source can be enriched using bovine corpus striatum as a source of receptor. This was subjected to subcellular fractionation and marker enzyme activities were estimated in resultant fractions. Receptor in membrane fractions was assayed by membrane bound radio receptor assay using [3H] diprenorphine as radioligand. The subcellular fractionation has achieved as enrichment of 2.36 fold in microsomes and 2.11 fold in synaptosomes over homogenate. The microsomal and synaptosomal fractions put together contribued to 13.3% of total protein and 30% of total receptors. These enriched memberans can be used as starting material for solubilization for higher yield of receptor.


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