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Year : 1995  |  Volume : 27  |  Issue : 2  |  Page : 106-110

A new approach in study of bioenergetics of macrofilariae using Nuclear magnetic resonance(NMR) spectroscopy

Correspondence Address:
A Shukla

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Source of Support: None, Conflict of Interest: None

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Nuclear magnetic resonance (NMR) offers new and exciting ways for examining biological phenomena in vivo and H, 13C and 31P NMR spectroscopy has been employed in metabolic studies on intact cells, tissues and organs as well as on whole organisms. In the present study 31PNMR studies were conducted to understand the effect of various drugs on the energy metabolism of filarial parasite. The 31PNMR at 22(C during perfusion with Ringer's solution saturated with N2 containing 5% CO: were composed of 12 peaks. Based on the chemical shift analysis of the intact parasites, the phosphorus component included sugar phosphates, nucleoside diphosphosugars, glycerophosphoryl choline, glycerophosphoryl ethanolamine, phosphoenolpyruvate and nucleotides mono, di and tri phosphates etc. Further standardisation of the perfusion method with normal adult filariids and male, female adult filarial worms separately has been carried out. In vivodrug administration using DEC (standard microfilaricidal drug for filariasis) at 250 mg/kg, i.p. x 5 days showed changes in the content of the 31PNMR peaks as compared with spectra of normal parasites.


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