| RESEARCH PAPER |
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| Year : 1994 | Volume
: 26
| Issue : 4 | Page : 288-291 |
Determination of buprenorphine and its N-dealkylated metabolite in urine TLC densitometry
Jain Raka
Correspondence Address:
Jain Raka
 Source of Support: None, Conflict of Interest: None  | Check |
A densitometric method was developed for determination of buprenorphine and its N-dealkylated metabolite (norbuprenorphine) in urine. The procedure involves acid hydrolysis followed by liquid - liquid extraction. The extracted residue was subjected to thin - layer chromatography and separation was achieved in two different solvent systems, Quantitation was done by scanning the sprayed plate at 500 nm using densitometer. The detection limit of the method is 0.5 (g\ ml. The method is simple, rapid, reproducible and suitable for analysis of urine samples taken from abusers of buprenorphine.
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