| RESEARCH PAPER
|Year : 1994 | Volume
| Issue : 2 | Page : 117-120
Bioassay for evaluation of the hepatoprotective effect of Liv.52, a Polyherbal formulation, on ethanol metabolism in chronic alcohol- Exposed rats
BL Chauhan, AR Mohan, RD Kulkarni, SK Mitra
A study was conducted on Wistar male rats to assess the effect of chronic alcohol administration on blood ethanol and acetaldehyde levels and their modification by Liv.52, an Ayurvedic formulation. Following chronic ingestion of 6% alcohol (v/v) through a water feeding bottle for 42 days, the administration of an acute oral dose of 5 ml of 5% alcohol (v/v) resulted in a significant decrease (P < 0.05) in blood ethanol and significant increase (P < 0.05) in acetaldehyde levels respectively at 30 min. Larger doses (i.e., 5 ml of 10% and 15% respectively) failed to demonstrate an effect of accelerated ethanol metabolism because of saturation kinetics. In the second experiment, temporal observations on blood ethanol and acetaldehyde levels were done at 1, 3 and 4 h. Following chronic ingestion of 6% alcohol for 42 days, stimulation of Phase I and suppression of Phase II metabolism was reproduced at all the time points, As compared with placebo, treatment with Liv.52 in the last two weeks reversed the blood ethanol and acetaldehyde levels (P < 0.005) at all the time points and they were comparable to the levels observed on day 1 (basal readings). In the third experiment, chronic exposure to 6% alcohol for 180 days showed further deterioration in ethanol metabolism as compared to 42 days exposure. In a chronic model of 180 days, Liv.52 normalised the blood ethanol and acetaldehyde levels in a dose- related manner. These observations are in agreement with our previous findings in chronic alcohol users. These bioassay findings could be effectively employed to evaluate the comparative efficacy of various polyherbal formulations on ethanol metabolism.
B L Chauhan
Source of Support: None, Conflict of Interest: None