| RESEARCH PAPER
|Year : 1987 | Volume
| Issue : 3 | Page : 174-178
Secondary structure of neuropeptide Y
V Renugopalakrishnan, A Balasubramaniam, JC Dobbs, LA Carreira, RS Rapaka
1, The synthesis, biological activity and secondary structure of nenropeptide Y and its 4-Norleu analogue are discussed, The neuropeptides were synthesized by step-wise solid phase synthesis.
2. Secondary structures in aqueous solutions were investigated by CD and Raman spectroscopy. Chou-Fasman predictive algorithm were indicative of a N-terminal B turn segment followed by eithe u-helical or p-sheet sub-domain, which was consistent with experimental studies.
3. 4-Norleu-neuropeptide Y was more potent in inhibiting heart pace maker activity and coronary flow but less potent in inhibiting the cardiac contractility than native neuropeptide Y. The importance of the 4 positions in NPY in the cardiac activity is suggested.
4. A tentative secondary structural model for neuropeptide Y is proposed from the above studies.
Source of Support: None, Conflict of Interest: None