| SHORT COMMUNICATION
|Year : 1987 | Volume
| Issue : 2 | Page : 140-144
Role of GABA in the anticonvulsant action of diazepam in albino mice
YV Rao, P Satishchandran, RP Kandlikar
1. Intraperitoneal diazepam (0.75-2.0 mg/kg) produced significant anticonvulsant effect in albino mice against pentylenetetrazol (80 mg/kg, SC) induced convulsions.
2. Pretreatment with thiosemicarbazide (5 mglkg, ip, 1 h), reduced the anticonvulsant effect of a sub-maximal dose of diazepam (1 mg/kg, ip, 2 h) whereas sodium valproate and muscimol (0,2 mg/kg, ip, 0.5 h) enhanced the effect of subanticonvulsant dose of diazepam (0.5 mg/kg, ip, 2 h) indicating the role of endogenous GABA in the effect of diazepam.
3. Biochemical studies also showed an increrse in cerebral cortical level of GABA in diazepam (1 mg/kg, ip, 2 h) or sodium valproate and diazepam (0.5 mg/kg, ip, 2 h) pretreated animals. Thiosemicarbazide pretreatment prevented the enhancement of cerebral cortical GABA level by diazepam (1 mg/kg, ip, 2 h).
Y V Rao
Source of Support: None, Conflict of Interest: None