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Year : 1984  |  Volume : 16  |  Issue : 4  |  Page : 206-216

Development of radioimmunoassay for digoxin

Correspondence Address:
M Bansinath

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Source of Support: None, Conflict of Interest: None

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1. Asensitiveand specific radioimmunossay (RIA) permitting quantitation of pharmacological levels of digoxin in human plasma has been developed. 2. High titer digoxin specific antibodies were obtained by immunizing rabbits with digoxin coupled by periodate oxidation method to bovine serum albumin. Anti-bodies produced by three animals have been extensively characterized regarding their suitability for RIA. 3. The assay involves use of tritiated digoxinand coated charcoal for separation of free and antibody bound radioactivity; assay is rapid and can readily cope with a large number of plasma samples and is adaptable for assay of digoxin in serum and urine samples. 4. The procedure has been evaluated by measuring plasma levels of digoxinin patients on digitalis therapy. Samples taken 12 hours after the last dose of digoxin showed a range of 0.36 to 3.14 ng/ml, with a mean value of 1.09 ( 0.8 ng/ml; the method is suitable for monitoring digoxin therapy.


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