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Year : 1983  |  Volume : 15  |  Issue : 2  |  Page : 95-100

An in vitro colorimetric method for assay of heparin sodium

Correspondence Address:
D E Mitali

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Source of Support: None, Conflict of Interest: None

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A simple and sensitive colorimetric method of assay of heparin sodium based on the comparative determination of anticoagulant activity of the test preparation with that of the standard has been deveIoped. The method involves the assessment of the quantity of heparin required to prevent 50 percent clotting (ED50) of a fixed amount of citrated goat plasma in presence of calcium ions. The method is more precise and rapid than and comparable in accuracy and reproducibility with the USP method. It has the further advantage that the subjectivity inherent in the existing bioassay methods is eliminated.


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