| RESEARCH PAPER
|Year : 1999 | Volume
| Issue : 6 | Page : 416-421
Evaluation of the hepatoprotective potential of jigrine pre-treatment on thioacetamide induced liver damage in rats
Ahmad Aftab, KK Pillai, Jameel Ahmed Shibli, DK Balani, AK Najmi, Marwah Renuka, Hameed Abdul
Objective: To evaluate the hepatoprotective potential of jigrine pre-treatment on thioacetamide induced liver damage in rats.
Methods: Albino rats (Wistar strain) were prophylactically treated with jigrine at two dose levels (0.5 ml and 1.0 ml/kg/day p.o.) for seven days. On days 6, rats were also given a single dose of thioacetamide (100 mg/ kg s.c.). Activity of jigrine against thioacetamide induced hepatotoxicity was also compared with silymarin (25 mg/kg/day p.o.). Biochemical parameters like serum AST, ALT, Na+ , K+ , tissue glutathione, tissue TBARS were estimated to assess the liver function. Biochemical observations were supplemented with histopathological examination of liver sections.
Results: Thioacetamide administration increased the AST, ALT, Na+ and K+ levels in serum and TBARS in liver. Pre-treatment of the rats with jigrine or silymarin reduced the thioacetamide induced elevated levels of the above indices. Thioacetamide administration decreased the concentration of GSH in rat liver. Jigrine pre-treatment at both the doses and silymarin restored the GSH levels to near normal values in the liver of rats treated with thioacetamide. Similarly the elevation in liver TBARS levels due to thiocetamide was prevented by silymarin and jigrine pretreaments. Thioacetamide administration induced centrilobular necrosis. Pre-treatment of the rats with jigrine at both the doses showed mild protection while silymarin showed marked protection on pre-treatment.
Conclusions: Jigrine pretreatment exhibited hepatoprotective action against thioacetamide induced toxicity. The effects of jigrine was comparable with that of silymarin.